Phosphoregulators

Previously, we have defined all protein kinases and protein phosphatases in mouse and set out to functionally annotate them. This resulted in the creation of the phosphoregulator database providing a summary of the sub-cellular localization, tissue distribution, classification, disease associations, substrates, ligands, interactions, nomenclature, and peptide sequence for each phosphoregulator locus.

>We are now using a systems biology approaches to model the phosphoregulator network in the normal cell cycle and/or tumour progression. This is being done by identifying all the expression pattern of all components of the network in normal and transformed cell types throughout the cell cycle and includes both canonical and variant phosphoregulators generated by alternative transcription events (see the phosphoregulator variant database for details on novel phosphoregulator isoforms).

We are also predicting and functionally screening key network components for an ability to regulate normal cell division. Finally we are also studying the role of alternate phosphoregulator promoter usage in the regulation of cell division and melanoma cell line progression.